A Flow Cytometric Method to Determine Transfection Efficiency

A novel rapid and reproducible flow cytometric method for optimization of transfection efficiency in cells

Transfection is one of the most frequently used techniques in molecular biology that is also applicable for gene therapy studies in humans. One of the biggest challenges to investigate the protein function and interaction in gene therapy studies is to have reliable monospecific detection reagents, particularly antibodies, for all human gene products. Thus, a reliable method that can optimize tr...

Inflammasome Formation A Novel Flow Cytometric Method To Assess

A pitfall of using a second plasmid to determine transfection efficiency.

When comparing the ability of two sequences to regulate the expression of a reporter gene, following transfection of cells in cultures, it is necessary to control for the potential difference in transfection efficiency. This is done by either conducting the experiment several times with at least two different preparations of each DNA or by adding a second plasmid (internal control plasmid) enco...

Flow cytometric method to isolate round spermatids from mouse testis.

The purpose of this study was to isolate pure populations of round spermatids from mouse testis by flow cytometry followed by cell sorting. Cell suspensions from mouse testis were enriched in germ cells by centrifugation on a discontinuous Percoll gradient, then analysed using a FACScalibur flow cytometer measuring the cell size and density. A large and well-delimited population of cells (R1) e...

A Fast Fluorimetric Flow Injection Method to Determine Ibuprofen

A fluorescência natural da ibuprofeína foi melhorada quando foi formado um complexo convidado-hospedeiro com a beta-ciclodextrina (beta-CD). A partir disso, foi desenvolvido um método por injeção em fluxo para a determinação de ibuprofeína em diferentes preparações farmacêuticas, com detecção fluorescente (λ em 287 nm, λ ex 233 nm). A curva de calibração foi linear no intervalo entre 6,00 a 60,...